In April of 2020 cuttings of Hashish sativa (L.) in a greenhouse in San Mateo County, CA had been noticed collapsing, and additional remark revealed: water-soaked stems, tan discoloration to the cortex, and discolored roots. The greenhouse irrigation system was provided by an area stream. We collected one-liter water samples from: consumption pond, reservoir tank, irrigation strains, and native potable water faucet. Water samples had been filtered and plated as described beforehand (Rollins et al., 2016). Filter papers had been eliminated after 24 hours. Crown sections from 4 symptomatic vegetation and one asymptomatic plant had been surfaced sterilized in 10% bleach for 5 minutes, rinsed in sterile deionized water, lower into four-millimeter lengthy sections, and plated onto V8 media, then incubated at room temperature for 3 days. White mycelial progress was noticed from foci throughout the print of the filter paper from all irrigation water samples however not the potable water provide pattern.
Comparable mycelial progress was noticed from plated crown tissue from symptomatic vegetation solely. Remark below gentle microscope revealed traits congruent with P. ultimum, together with aseptate hyphae and globose sporangia (Watanabe, 2002). Mycelia was collected for DNA extraction from every of the water and plant pattern plates with DNA extractions carried out utilizing Fast DNA Fungi/Bacterial Equipment (Zymo Analysis Irvine, CA, USA) and PCR amplified utilizing primers ITS100/ITS4 as described by Riit et al. (2016). All amplicons had been Sanger sequenced, aligned utilizing SnapGene software program (from GSL Biotech; out there at snapgene.com), and in comparison with barcode referencPe sequences to establish the species utilizing the BarCode of Life Database (BOLDsystems) throughout the Nationwide Heart for Biotechnology Data nucleotide database.
After trimming and aligning, all amplicons had been discovered to be equivalent, yielding the 810-nucleotide lengthy consensus ITS amplicon (accession MW114807), which aligned with Pythium ultimum ITS sequences (e.g., accession HQ643886.1) with 100% id and homology. We then accomplished Koch’s postulates through the use of pure cultures from root sections of P. ultimum to stem inoculate C. sativa vegetation. We used a three-millimeter corer to take away a disc of dermis and utilized a plug of pure tradition to the wound. We inoculated 10 vegetation, with two vegetation mock-inoculated utilizing clear V8 agar. Inoculation websites had been wrapped in parafilm, and vegetation had been grown within the greenhouse for 20 days. Stems of mock and oomycete inoculated vegetation had been examined for callus formation and 30 centimeters of stem had been excised from every plant. The mock inoculated vegetation had totally callused inoculation websites and had been discolored solely the place wounded.
P. ultimum inoculated plant inoculation websites had been partially callused over and had tan discoloration of the cortex that prolonged 6.zero mm +/- 2.zero mm above and beneath the inoculation website. Stem segments above and beneath inoculation websites had been floor sterilized and plated on V8 media as beforehand described and P. ultimum recovered from inoculated vegetation, confirmed as equivalent to the inoculum by ITS amplification and sequencing. Mock inoculated plant stem cultures yielded no oomycete progress. Collectively, these outcomes point out that P. ultimum has the power to trigger crown rot in C. sativa in greenhouse cultivation.

The evolution of polymer conjugation and drug concentrating on for the supply of proteins and bioactive molecules
Steering the Personal Sector in COVID-19 Diagnostic Check Equipment Growth in South Korea
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BSH1001-E | Benchmark Scientific | 1 PC | EUR 625.06 |
Two-Block Digital Dry Bath |
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BSH1002-E | Benchmark Scientific | 1 PC | EUR 997.76 |
Four-Block Digital Dry Bath |
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BSH1004-E | Benchmark Scientific | 1 PC | EUR 182.32 |
One-Block Digital Dry Bath 115V |
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BCM1412 | Bio Basic | 1 pcs, 1 UNIT | EUR 739.7 |
Two-Block Digital Dry Bath 115V |
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Four-Block Digital Dry Bath 115V |
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BCM1414 | Bio Basic | 1 pcs, 1 UNIT | EUR 1423 |
Digital Dry Bath Heater Single Block |
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6877-SB | Scientific Laboratory Supplies | EACH | EUR 429.78 |
Four-Block Digital Dry Bath 230V |
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BLO1272 | Scientific Laboratory Supplies | EACH | EUR 1178.76 |
Flowgen Digital Dry Bath Single Position |
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BLO2250 | Scientific Laboratory Supplies | EACH | EUR 624.72 |
Flowgen Digital Dry Bath Dual Position |
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BLO2252 | Scientific Laboratory Supplies | EACH | EUR 779.76 |
One-Block Digital Dry Bath 115V |
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BSH1001 | Benchmark Scientific | 1 each | EUR 535.9 |
Two-Block Digital Dry Bath 115V |
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BSH1002 | Benchmark Scientific | 1 each | EUR 668.4 |
Four-Block Digital Dry Bath 115V |
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BSH1004 | Benchmark Scientific | 1 each | EUR 1080.3 |
Myblock Mini Dry Bath Block Holds |
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Z680052-1EA | Scientific Laboratory Supplies | EACH | EUR 108.3 |
DIGITAL DRY BATH HEATER, SINGLE BLOCK,120V |
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6875-SB | CORNING | 1/pk | EUR 414 |
Description: Lab Equipment; Constant Temperature Equipment |
DIGITAL DRY BATH HEATER, DUAL BLOCK,120V |
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6885-DB | CORNING | 1/pk | EUR 483.6 |
Description: Lab Equipment; Constant Temperature Equipment |
DIGITAL DRY BATH HEATER, FOUR BLOCK,120V |
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6895-FB | CORNING | 1/pk | EUR 696 |
Description: Lab Equipment; Constant Temperature Equipment |
Mini Dry Bath, 100-240V (US Plug) |
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BCM1405 | Bio Basic | 1 pcs, 1 UNIT | EUR 473.48 |
Block 24x1.5ml for Flowgen Digital Dry Bath |
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BLO2254 | Scientific Laboratory Supplies | EACH | EUR 159.6 |
Block 24x0.5ml for Flowgen Digital Dry Bath |
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BLO2258 | Scientific Laboratory Supplies | EACH | EUR 159.6 |
Block 12x15ml for Flowgen Digital Dry Bath |
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BLO2260 | Scientific Laboratory Supplies | EACH | EUR 159.6 |
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Block 20x10mm for Flowgen Digital Dry Bath |
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Block 20x13ml for Flowgen Digital Dry Bath |
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BLO2266 | Scientific Laboratory Supplies | EACH | EUR 159.6 |
digital dry bath, single chamber, without blocks, 115V |
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BCM1407 | Bio Basic | 1 pcs, 1 UNIT | EUR 739.7 |
digital dry bath, dual chamber, without blocks, 115V |
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Block, PCR plate, For 1-block dry bath |
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Block 12x15-16mm for Flowgen Digital Dry Bath |
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Myblock Mini Dry Bath. 100-240 V. W |
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Z680001-1EA | Scientific Laboratory Supplies | EACH | EUR 287.28 |
DIGITAL DRY BATH HEATER, DUAL BLOCK,230V-EU PLUG |
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6786-DB | CORNING | 1/pk | EUR 483.6 |
Description: Lab Equipment; Constant Temperature Equipment |
DIGITAL DRY BATH HEATER, SINGLE BLOCK,230V-EU PLUG |
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6786-SB | CORNING | 1/pk | EUR 414 |
Description: Lab Equipment; Constant Temperature Equipment |
DIGITAL DRY BATH HEATER, DUAL BLOCK,230V-UK PLUG |
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6787-DB | CORNING | 1/pk | EUR 483.6 |
Description: Lab Equipment; Constant Temperature Equipment |
DIGITAL DRY BATH HEATER, SINGLE BLOCK,230V-UK PLUG |
|||
6787-SB | CORNING | 1/pk | EUR 414 |
Description: Lab Equipment; Constant Temperature Equipment |
DIGITAL DRY BATH HEATER, SINGLE BLOCK,230V-EU PLUG |
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6876-SB | CORNING | 1/pk | EUR 414 |
Description: Lab Equipment; Constant Temperature Equipment |
DIGITAL DRY BATH HEATER, DUAL BLOCK,230V-EU PLUG |
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6886-DB | CORNING | 1/pk | EUR 483.6 |
Description: Lab Equipment; Constant Temperature Equipment |
DIGITAL DRY BATH HEATER, FOUR BLOCK,230V-EU PLUG |
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6896-FB | CORNING | 1/pk | EUR 696 |
Description: Lab Equipment; Constant Temperature Equipment |
myBlock l- digi dry bath with 1 BLO1280 230V |
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BLO1264 | Scientific Laboratory Supplies | EACH | EUR 715.92 |
myBlock ll- digi dry bath with 2 BLO1280 230V |
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BLO1266 | Scientific Laboratory Supplies | EACH | EUR 1053.36 |
Block 24x1.5ml or 2.0ml for Flowgen Digital Dry Bath |
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BLO2256 | Scientific Laboratory Supplies | EACH | EUR 159.6 |
MyBlock™ Mini Dry Bath, 100-240V (US Plug) |
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BSH200 | Benchmark Scientific | 1 each | EUR 309.1 |
24 x 0.2mL block, accessory for DualBloc? Dry Bath |
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DDBI-CL-96-A | ACTGene | each | EUR 225.6 |
12 x 0.5mL block, accessory for DualBloc? Dry Bath |
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6 x 1.5mL block, accessory for DualBloc? Dry Bath |
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6 x 2.0mL block, accessory for DualBloc? Dry Bath |
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96 x 0.2mL block, accessory for DualBloc? Dry Bath |
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6 x 15mL block, accessory for DualBloc? Dry Bath |
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DDBI-CL-96-F | ACTGene | each | EUR 313.2 |
3 x 50mL block, accessory for DualBloc? Dry Bath |
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DDBI-CL-96-G | ACTGene | each | EUR 313.2 |
Palm?Dry Bath Incubator Heating (5 to 100 ?) main unit only, blocks all optional, portable with optional car charger |
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ACT-PALM-24HT | ACTGene | each | EUR 790.8 |
Block, PCR plate, for 2 or 4-block dry bath |
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MyBlock HL Mini Dry Bath with Heated Lid 100-240V |
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BLO1236 | Scientific Laboratory Supplies | EACH | EUR 533.52 |
MyBlock Mini dry bath with Heating and Cooling 100-240V |
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BLO1238 | Scientific Laboratory Supplies | EACH | EUR 866.4 |
myBlock l- digital dry bath single chamber without blocks 230V |
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myBlock ll- digital dry bath dual chamber without blocks 230V |
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BLO1262 | Scientific Laboratory Supplies | EACH | EUR 738.72 |
Car charger, accessory for Palm? Dry Bath, accessory for Palm? |
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ACT-PALM-24G | ACTGene | each | EUR 168 |
HL Mini Dry Bath with Heated Lid, 100-240V (US Plug) |
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BCM1406 | Bio Basic | 1 pcs, 1 UNIT | EUR 695.33 |
digital dry bath, with two independently controlled chambers, without blocks, 115V |
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BCM1411 | Bio Basic | 1 pcs, 1 UNIT | EUR 1174.52 |
Corning LSE Digital Dry Bath Heater Dual Block 230V UK Plug |
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6887-DB | Scientific Laboratory Supplies | EACH | EUR 581.4 |
Corning LSE Digital dry bath heater four block 230V UK Plug |
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6897-FB | Scientific Laboratory Supplies | EACH | EUR 763.8 |
Block 48x0.2ml Or 6 PCR Strips for Flowgen Digital Dry Bath |
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MyBlock™ Mini dry bath with cooling, 100-240V (US Plug) |
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BSH300 | Benchmark Scientific | 1 each | EUR 817.6 |
myBlock l™ - digital dry bath, single chamber, without blocks, 115V |
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myBlock ll™ - digital dry bath, dual chamber, without blocks, 115V |
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Block, 15 x 1.5ml Centrifuge Tubes for Dry Bath with Heated Lid |
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Block 15 x 1.5mL Centrifuge Tubes for Dry Bath with Heated Lid |
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isoBlock digi dry bath w 2 indep controlled chamb w/ blocks 230V |
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IKA RV 8 Rotary Evaporator with Heating Bath and Dry Ice Condenser |
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IKA RV 3 Rotary Evaporator with Heating Bath and Dry Ice Condenser |
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Heating Block for Block Flowgen Mini Digital Dry Bath 15x1.5 or 2.0ml tubes |
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IKA RV 8 Rotary Evaporator with Heating Bath and Coated Dry Ice Condenser |
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IKA RV 10 digital Rotary Evaporator with Heating Bath and Dry Ice Condenser |
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IKA RV 3 Rotary Evaporator with Heating Bath and Coated Dry Ice Condenser |
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MyBlock™ HL Mini Dry Bath with Heated Lid, 100-240V (US Plug) |
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BSH200-HL | Benchmark Scientific | 1 each | EUR 507.3 |
isoBlock™ - digital dry bath, with two independently controlled chambers, without blocks, 115V |
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TB Dry |
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A2536-1000000 | ApexBio | 1 kg | EUR 351.6 |
Description: TB Dry is a single component high nutrient medium formulatedto maximize yields of recombinant plasmids and cosmids.The most important advantage of TB DRY is that all componentsare combined into a single powder, eliminating the need to autoclave two separate solutions. |
TB Dry |
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A2536-5000000 | ApexBio | 5 kg | EUR 1432.8 |
Description: TB Dry is a single component high nutrient medium formulatedto maximize yields of recombinant plasmids and cosmids.The most important advantage of TB DRY is that all componentsare combined into a single powder, eliminating the need to autoclave two separate solutions. |
Dionex Ultimate 3000 Tungsten Lamp |
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LT-DIO-105 | Scientific Laboratory Supplies | EACH | EUR 319.2 |
Incubator IN30 |
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INC8138 | Scientific Laboratory Supplies | EACH | EUR 1440.96 |
Incubator IN55 |
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Incubator IN750 |
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Incubator IN30plus |
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Incubator IF30 |
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INC8170 | Scientific Laboratory Supplies | EACH | EUR 1721.4 |
Incubator IF55 |
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INC8174 | Scientific Laboratory Supplies | EACH | EUR 2504.58 |
Incubator IF110 |
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INC8176 | Scientific Laboratory Supplies | EACH | EUR 2749.68 |
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INC8186 | Scientific Laboratory Supplies | EACH | EUR 2240.1 |
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INC8188 | Scientific Laboratory Supplies | EACH | EUR 2675.58 |
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INC8192 | Scientific Laboratory Supplies | EACH | EUR 3393.78 |
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INC8194 | Scientific Laboratory Supplies | EACH | EUR 4229.4 |
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INC8196 | Scientific Laboratory Supplies | EACH | EUR 5114.04 |
Incubator IF450plus |
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INC8198 | Scientific Laboratory Supplies | EACH | EUR 6549.3 |
Incubator IF750plus |
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INC8200 | Scientific Laboratory Supplies | EACH | EUR 8576.22 |
Block Micro Titer Plate skirted/non f 2 or 4-block dry bath only |
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BLO1282 | Scientific Laboratory Supplies | EACH | EUR 261.06 |
Block 1x96 Well PCR plate Single Block Unit Only for Flowgen Digital Dry Bath |
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BLO2274 | Scientific Laboratory Supplies | EACH | EUR 356.82 |
IKA RV 10 digital Rotary Evaporator with Heating Bath and Coated Dry Ice Condenser |
|||
EVA3116 | Scientific Laboratory Supplies | EACH | EUR 6067.08 |
DualBloc? Cooling/Heating (-10 to 100 degrees) Dry Bath, Main unit only, Blocks optional |
|||
DDBI-CL-96 | ACTGene | each | EUR 2952 |
Block 1x96 Well PCR Plate For Double Block Unit Only for Flowgen Digital Dry Bath |
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BLO2272 | Scientific Laboratory Supplies | EACH | EUR 344.28 |
Dry Anaerobic Indstrip |
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271051 | Scientific Laboratory Supplies | PK100 | EUR 58.14 |
CL4 Wipers Dry |
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CLE3400 | Scientific Laboratory Supplies | PK5 | EUR 320.34 |
CL4 Wipers Dry |
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CLE3402 | Scientific Laboratory Supplies | PK5 | EUR 175.56 |
CX100B Dry Shipper |
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CRY7066 | Scientific Laboratory Supplies | EACH | EUR 1235.76 |
CXR100 Dry Shipper |
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CRY7068 | Scientific Laboratory Supplies | EACH | EUR 1448.94 |
Dry DMSO, 14ML |
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X022-14ML | Arbor Assays | 14ML | EUR 171.6 |
Dry DMSO, 20ML |
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X022-20ML | Arbor Assays | 20ML | EUR 232.8 |
Dry DMSO, 2ML |
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X022-2ML | Arbor Assays | 2ML | EUR 112.8 |
Dry DMSO, 4ML |
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X022-4ML | Arbor Assays | 4ML | EUR 139.2 |
Dry DMSO, 5ML |
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NEDD8 Ultimate Buster 1 (NUB1) Antibody |
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20-abx008140 | Abbexa |
|
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NEDD8 Ultimate Buster 1 (NUB1) Antibody |
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20-abx124654 | Abbexa |
|
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NEDD8 ultimate buster 1 (NUB1) Antibody |
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abx331552-100ul | Abbexa | 100 ul | EUR 510 |
NEDD8 ultimate buster 1 (NUB1) Antibody |
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20-abx326307 | Abbexa |
|
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NEDD8 Ultimate Buster 1 (NUB1) Antibody |
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abx235887-100ug | Abbexa | 100 ug | EUR 577.2 |
CORIO C-B5 5L Bath Circulator SS Bath |
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BAT8111 | Scientific Laboratory Supplies | EACH | EUR 1394.22 |
CORIO C-B13 13L Bath Circulator SS Bath |
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BAT8112 | Scientific Laboratory Supplies | EACH | EUR 1653 |
CORIO C-B17 17L Bath Circulator SS Bath |
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CORIO C-B27 27L Bath Circulator SS Bath |
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CORIO CD-B5 5L Bath Circ. SS Bath |
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CORIO CD-B19 19L Bath Circ. SS Bath |
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CORIO CD-B27 27L Bath Circ. SS Bath |
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CORIO CD-B33 33L Bath Circ. SS Bath |
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CORIO CD-BC4 4.5L Bath Circ. SS Bath |
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CORIO CD-BC6 6L Bath Circ. SS Bath |
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CORIO CD-BC12 12L Bath Circ. SS Bath |
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CORIO CD-BC26 26L Bath Circ. SS Bath |
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BAT8129 | Scientific Laboratory Supplies | EACH | EUR 3386.94 |
Ultraclean Bath Detergent |
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BAT2030 | Scientific Laboratory Supplies | EACH | EUR 16.17 |
Digital Bath - 4L |
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Digital Bath - 8L |
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Digital Bath - 14L |
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BAT2840 | Scientific Laboratory Supplies | EACH | EUR 508.44 |
Digital Bath - 22L |
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BAT2842 | Scientific Laboratory Supplies | EACH | EUR 614.46 |
Pipette Bath 10cx55cm |
|||
WAS3130 | Scientific Laboratory Supplies | EACH | EUR 96.9 |
Block, Micro Titer Plate, skirted or non-skirted For 2 or 4-block dry bath only |
|||
BCM1412-BSWMT | Bio Basic | 1 pcs, 1 UNIT | EUR 338.22 |
Shaking Incubator 49L |
|||
6752 | Scientific Laboratory Supplies | EACH | EUR 5147.1 |
Shaking Incubator 71L |
|||
6754 | Scientific Laboratory Supplies | EACH | EUR 5723.94 |
Incubator Dipslide DI10 |
|||
INC1012 | Scientific Laboratory Supplies | EACH | EUR 437.76 |
Incubator BD056-230V |
|||
INC1015 | Scientific Laboratory Supplies | EACH | EUR 1553.82 |
Incubator BD260-230V |
|||
INC1016 | Scientific Laboratory Supplies | EACH | EUR 3540.84 |
Incubator ED056-230V |
|||
INC1017 | Scientific Laboratory Supplies | EACH | EUR 1346.34 |