In April of 2020 cuttings of Hashish sativa (L.) in a greenhouse in San Mateo County, CA had been noticed collapsing, and additional remark revealed: water-soaked stems, tan discoloration to the cortex, and discolored roots. The greenhouse irrigation system was provided by an area stream. We collected one-liter water samples from: consumption pond, reservoir tank, irrigation strains, and native potable water faucet. Water samples had been filtered and plated as described beforehand (Rollins et al., 2016). Filter papers had been eliminated after 24 hours. Crown sections from 4 symptomatic vegetation and one asymptomatic plant had been surfaced sterilized in 10% bleach for 5 minutes, rinsed in sterile deionized water, lower into four-millimeter lengthy sections, and plated onto V8 media, then incubated at room temperature for 3 days. White mycelial progress was noticed from foci throughout the print of the filter paper from all irrigation water samples however not the potable water provide pattern.
Comparable mycelial progress was noticed from plated crown tissue from symptomatic vegetation solely. Remark below gentle microscope revealed traits congruent with P. ultimum, together with aseptate hyphae and globose sporangia (Watanabe, 2002). Mycelia was collected for DNA extraction from every of the water and plant pattern plates with DNA extractions carried out utilizing Fast DNA Fungi/Bacterial Equipment (Zymo Analysis Irvine, CA, USA) and PCR amplified utilizing primers ITS100/ITS4 as described by Riit et al. (2016). All amplicons had been Sanger sequenced, aligned utilizing SnapGene software program (from GSL Biotech; out there at snapgene.com), and in comparison with barcode referencPe sequences to establish the species utilizing the BarCode of Life Database (BOLDsystems) throughout the Nationwide Heart for Biotechnology Data nucleotide database.
After trimming and aligning, all amplicons had been discovered to be equivalent, yielding the 810-nucleotide lengthy consensus ITS amplicon (accession MW114807), which aligned with Pythium ultimum ITS sequences (e.g., accession HQ643886.1) with 100% id and homology. We then accomplished Koch’s postulates through the use of pure cultures from root sections of P. ultimum to stem inoculate C. sativa vegetation. We used a three-millimeter corer to take away a disc of dermis and utilized a plug of pure tradition to the wound. We inoculated 10 vegetation, with two vegetation mock-inoculated utilizing clear V8 agar. Inoculation websites had been wrapped in parafilm, and vegetation had been grown within the greenhouse for 20 days. Stems of mock and oomycete inoculated vegetation had been examined for callus formation and 30 centimeters of stem had been excised from every plant. The mock inoculated vegetation had totally callused inoculation websites and had been discolored solely the place wounded.
P. ultimum inoculated plant inoculation websites had been partially callused over and had tan discoloration of the cortex that prolonged 6.zero mm +/- 2.zero mm above and beneath the inoculation website. Stem segments above and beneath inoculation websites had been floor sterilized and plated on V8 media as beforehand described and P. ultimum recovered from inoculated vegetation, confirmed as equivalent to the inoculum by ITS amplification and sequencing. Mock inoculated plant stem cultures yielded no oomycete progress. Collectively, these outcomes point out that P. ultimum has the power to trigger crown rot in C. sativa in greenhouse cultivation.
The evolution of polymer conjugation and drug concentrating on for the supply of proteins and bioactive molecules
Steering the Personal Sector in COVID-19 Diagnostic Check Equipment Growth in South Korea
MycGuard™-1 Solution (100×), for Disinfecting Water Bath of CO2 Incubator |
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40609ES60 | Yeasen Biotechnology | 100 mL | EUR 145 |
CO2 Incubator, 150 L |
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Q5103 | ABM | 1 | EUR 16500 |
Incubator IN30 - EACH |
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INC8138 | Scientific Laboratory Supplies | EACH | EUR 1960.2 |
Incubator IN55 - EACH |
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INC8140 | Scientific Laboratory Supplies | EACH | EUR 2442.15 |
Incubator IN75 - EACH |
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INC8142 | Scientific Laboratory Supplies | EACH | EUR 2427.3 |
Incubator IF30 - EACH |
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INC8170 | Scientific Laboratory Supplies | EACH | EUR 2332.8 |
Incubator IF55 - EACH |
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INC8172 | Scientific Laboratory Supplies | EACH | EUR 2706.75 |
Incubator IF75 - EACH |
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INC8174 | Scientific Laboratory Supplies | EACH | EUR 3335.85 |
Ultimate C |
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E4A10A38U | EnoGene | 50ug | EUR 255 |
Description: Available in various conjugation types. |
Incubator IN110 - EACH |
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INC8144 | Scientific Laboratory Supplies | EACH | EUR 2862 |
Incubator IN260 - EACH |
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INC8148 | Scientific Laboratory Supplies | EACH | EUR 5073.3 |
Incubator IN450 - EACH |
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INC8150 | Scientific Laboratory Supplies | EACH | EUR 6704.1 |
Incubator IN750 - EACH |
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INC8152 | Scientific Laboratory Supplies | EACH | EUR 8896.5 |
Incubator IF110 - EACH |
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INC8176 | Scientific Laboratory Supplies | EACH | EUR 3661.2 |
Incubator IF160 - EACH |
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INC8178 | Scientific Laboratory Supplies | EACH | EUR 4496.85 |
Incubator IF260 - EACH |
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INC8180 | Scientific Laboratory Supplies | EACH | EUR 5840.1 |
Incubator IF450 - EACH |
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INC8182 | Scientific Laboratory Supplies | EACH | EUR 7519.5 |
Incubator IF750 - EACH |
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INC8184 | Scientific Laboratory Supplies | EACH | EUR 9680.85 |
UVP Incubator, 230V UK + EU |
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AJ95-0358-02 | Westburg | each | EUR 3498.9 |
SureTherm CO2 Incubator, 180L |
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H3565-180 | Benchmark Scientific | 1 each | EUR 7470.8 |
Crystal Growth Incubator 285L |
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M-CI-IS26SD/PC | MiTeGen | 1 UNIT | EUR 7175 |
Description: Crystal Growth Incubator 285L |
Crystal Growth Incubator 680L |
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M-CI-IS28SSD/PC | MiTeGen | 1 UNIT | EUR 7885 |
Description: Crystal Growth Incubator 680L |
Crystal Growth Incubator 765L |
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M-CI-IS33SD/PC | MiTeGen | 1 UNIT | EUR 8960 |
Description: Crystal Growth Incubator 765L |
Incubator IN30plus - EACH |
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INC8154 | Scientific Laboratory Supplies | EACH | EUR 2771.55 |
Incubator IN55plus - EACH |
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INC8156 | Scientific Laboratory Supplies | EACH | EUR 3125.25 |
Incubator IN75plus - EACH |
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INC8158 | Scientific Laboratory Supplies | EACH | EUR 3527.55 |
Incubator IF30plus - EACH |
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INC8186 | Scientific Laboratory Supplies | EACH | EUR 2982.15 |
Incubator IF55plus - EACH |
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INC8188 | Scientific Laboratory Supplies | EACH | EUR 3562.65 |
Incubator IF75plus - EACH |
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INC8190 | Scientific Laboratory Supplies | EACH | EUR 4012.2 |
Immunofluorescence Incubator |
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FIA-101 | CTK Biotech | each | EUR 5335 |
Incubator IN110plus - EACH |
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INC8160 | Scientific Laboratory Supplies | EACH | EUR 3960.9 |
Incubator IN160plus - EACH |
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INC8162 | Scientific Laboratory Supplies | EACH | EUR 4941 |
Incubator IN260plus - EACH |
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INC8164 | Scientific Laboratory Supplies | EACH | EUR 6018.3 |
Incubator IN450plus - EACH |
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INC8166 | Scientific Laboratory Supplies | EACH | EUR 7824.6 |
Incubator IN750plus - EACH |
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INC8168 | Scientific Laboratory Supplies | EACH | EUR 10066.95 |
Incubator IF110plus - EACH |
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INC8192 | Scientific Laboratory Supplies | EACH | EUR 4517.1 |
Incubator IF160plus - EACH |
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INC8194 | Scientific Laboratory Supplies | EACH | EUR 5629.5 |
Incubator IF260plus - EACH |
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INC8196 | Scientific Laboratory Supplies | EACH | EUR 6808.05 |
Incubator IF450plus - EACH |
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INC8198 | Scientific Laboratory Supplies | EACH | EUR 8718.3 |
Incubator IF750plus - EACH |
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INC8200 | Scientific Laboratory Supplies | EACH | EUR 11206.35 |
UniPact? Shaking Incubator |
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ACT-USI | ACTGene | each | EUR 5560.8 |
SureTherm CO2 Incubator, 180L, 230V |
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H3565-180-E | Benchmark Scientific | each | EUR 7470.8 |
Binder BD115 Incubator 115L - EACH |
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INC3004 | Scientific Laboratory Supplies | EACH | EUR 2693.78 |
Binder BD400 Incubator 400L - EACH |
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INC3008 | Scientific Laboratory Supplies | EACH | EUR 6527.35 |
SHAKING INCUBATOR, 49L, 120V |
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6750 | CORNING | 1/pk | EUR 4740 |
Description: Lab Equipment; Constant Temperature Equipment |
SHAKING INCUBATOR, 71L, 120V |
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6753 | CORNING | 1/pk | EUR 5392.8 |
Description: Lab Equipment; Constant Temperature Equipment |
Shaking Incubator 49L - EACH |
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6752 | Scientific Laboratory Supplies | EACH | EUR 6339.6 |
Shaking Incubator 71L - EACH |
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6754 | Scientific Laboratory Supplies | EACH | EUR 7049.7 |
ComfyTempTM Portable Incubator |
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CTPI-300 | Avans Biotechnology Corp | each | EUR 1100 |
SureTherm CO2 Incubator, 45L |
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H3565-45 | Benchmark Scientific | 1 each | EUR 6184.74 |
Incubator BD056-230V - EACH |
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INC1015 | Scientific Laboratory Supplies | EACH | EUR 2001.97 |
Incubator BD260-230V - EACH |
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INC1016 | Scientific Laboratory Supplies | EACH | EUR 4562.09 |
Incubator ED056-230V - EACH |
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INC1017 | Scientific Laboratory Supplies | EACH | EUR 1887.41 |
Incubator BF056-230V - EACH |
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INC1019 | Scientific Laboratory Supplies | EACH | EUR 2718.75 |
Incubator BF260-230V - EACH |
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INC1021 | Scientific Laboratory Supplies | EACH | EUR 5525.63 |
LMS Incubator 120L Series 1A - EACH |
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INC5302 | Scientific Laboratory Supplies | EACH | EUR 4691.25 |
LMS Incubator 201L Series 1A - EACH |
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INC5304 | Scientific Laboratory Supplies | EACH | EUR 4981.5 |
LMS Incubator 272L Series 1A - EACH |
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INC5306 | Scientific Laboratory Supplies | EACH | EUR 5134.05 |
Multipurpose Incubator 100C 250L - EACH |
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INC2262 | Scientific Laboratory Supplies | EACH | EUR 6218.1 |
Multipurpose Incubator 100C 320L - EACH |
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INC2264 | Scientific Laboratory Supplies | EACH | EUR 6551.55 |
Multipurpose Incubator 100C 450L - EACH |
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INC2266 | Scientific Laboratory Supplies | EACH | EUR 8240.4 |
Multipurpose Incubator 100C 535L - EACH |
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INC2268 | Scientific Laboratory Supplies | EACH | EUR 8924.85 |
Multipurpose Incubator 100C 645L - EACH |
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INC2270 | Scientific Laboratory Supplies | EACH | EUR 12098.7 |
Multipurpose Incubator 100C 750L - EACH |
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INC2272 | Scientific Laboratory Supplies | EACH | EUR 11040.3 |
Multipurpose Incubator 100C 865L - EACH |
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INC2274 | Scientific Laboratory Supplies | EACH | EUR 13026.15 |
Compressor cooled Incubator -256 - EACH |
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INC2400 | Scientific Laboratory Supplies | EACH | EUR 14040 |
Compressor cooled Incubator -449 - EACH |
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INC2402 | Scientific Laboratory Supplies | EACH | EUR 17219.25 |
Compressor cooled Incubator -749 - EACH |
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INC2404 | Scientific Laboratory Supplies | EACH | EUR 19620.9 |
Incubator Dipslide DI10 - EACH |
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INC1012 | Scientific Laboratory Supplies | EACH | EUR 581.85 |
Digital Incubator S160D - EACH |
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INC7210 | Scientific Laboratory Supplies | EACH | EUR 3636.9 |
Mini Incubator for Test Kit |
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M1005 | Ring Biotechnology Co | 1set | EUR 100 |
Description: Instrument |
92 L Cooled Incubator epoxy |
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INC2156 | Scientific Laboratory Supplies | EACH | EUR 3954.66 |
Refrigerated Cooled Incubator 150L - EACH |
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RLSD01503 | Scientific Laboratory Supplies | EACH | EUR 5335.2 |
Refrigerated Cooled Incubator 340L - EACH |
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RLSD03401 | Scientific Laboratory Supplies | EACH | EUR 6309.9 |
Refrigerated Cooled Incubator 570L - EACH |
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RLSD0600 | Scientific Laboratory Supplies | EACH | EUR 11853 |
LEEC C100 Classic Incubator 100L - EACH |
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INC1900 | Scientific Laboratory Supplies | EACH | EUR 2980.8 |
BF115 Incubator Mech 115L - EACH |
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INC3036 | Scientific Laboratory Supplies | EACH | EUR 3550.09 |
BF400 Incubator Mech 400L - EACH |
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INC3038 | Scientific Laboratory Supplies | EACH | EUR 6904.83 |
BF720 Incubator Mech 720L - EACH |
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INC3040 | Scientific Laboratory Supplies | EACH | EUR 9221.13 |
92 L Cooled Incubator St S |
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INC2172 | Scientific Laboratory Supplies | EACH | EUR 4433.46 |
92 L Cooled Incubator St S |
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INC2180 | Scientific Laboratory Supplies | EACH | EUR 4664.88 |
LMS Cooled Incubator 1200L Series 4 - EACH |
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INC5022 | Scientific Laboratory Supplies | EACH | EUR 11637.3 |
Benchtop Incubator Sts - 6L - EACH |
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INC2028 | Scientific Laboratory Supplies | EACH | EUR 1506.6 |
Binder BD23 Incubator 23L - EACH |
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INC3000 | Scientific Laboratory Supplies | EACH | EUR 2065.13 |
294 L Cooled Incubator St S |
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INC2176 | Scientific Laboratory Supplies | EACH | EUR 5515.32 |
516 L Cooled Incubator St S |
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INC2178 | Scientific Laboratory Supplies | EACH | EUR 7348.44 |
294 L Cooled Incubator St S |
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INC2184 | Scientific Laboratory Supplies | EACH | EUR 6184.5 |
516 L Cooled Incubator St S |
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INC2186 | Scientific Laboratory Supplies | EACH | EUR 8099.7 |
SureTherm CO2 Incubator, 45L, 230V |
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H3565-45-E | Benchmark Scientific | each | EUR 6184.74 |
Benchtop Incubator Sts - 18L - EACH |
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INC2030 | Scientific Laboratory Supplies | EACH | EUR 1591.65 |
Benchtop Incubator Sts - 30L - EACH |
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INC2032 | Scientific Laboratory Supplies | EACH | EUR 1567.35 |
Benchtop Incubator Sts - 40L - EACH |
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INC2034 | Scientific Laboratory Supplies | EACH | EUR 1715.85 |
Benchtop Incubator Sts - 50L - EACH |
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INC2036 | Scientific Laboratory Supplies | EACH | EUR 1836 |
Benchtop Incubator Sts - 75L - EACH |
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INC2038 | Scientific Laboratory Supplies | EACH | EUR 2050.65 |
Benchtop Incubator Sts - 100L - EACH |
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INC2040 | Scientific Laboratory Supplies | EACH | EUR 2263.95 |
Benchtop Incubator Sts - 125L - EACH |
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INC2042 | Scientific Laboratory Supplies | EACH | EUR 2289.6 |
Benchtop Incubator Sts - 150L - EACH |
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INC2044 | Scientific Laboratory Supplies | EACH | EUR 2254.5 |
Benchtop Incubator Sts - 200L - EACH |
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INC2046 | Scientific Laboratory Supplies | EACH | EUR 3264.3 |
Flystuff Ultimate Flypad |
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59-172 | Genesee Scientific | 1 Flypad/Unit | EUR 455.97 |
Description: With Accessories |
Benchtop Incubator CLAD - 18L - EACH |
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INC2010 | Scientific Laboratory Supplies | EACH | EUR 1502.55 |
Benchtop Incubator CLAD - 30L - EACH |
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INC2012 | Scientific Laboratory Supplies | EACH | EUR 1591.65 |
Benchtop Incubator CLAD - 40L - EACH |
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INC2014 | Scientific Laboratory Supplies | EACH | EUR 1594.35 |
Benchtop Incubator CLAD - 50L - EACH |
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INC2016 | Scientific Laboratory Supplies | EACH | EUR 1699.65 |
Benchtop Incubator CLAD - 75L - EACH |
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INC2018 | Scientific Laboratory Supplies | EACH | EUR 1895.4 |
Benchtop Incubator CLAD - 100L - EACH |
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INC2020 | Scientific Laboratory Supplies | EACH | EUR 2091.15 |
Benchtop Incubator CLAD - 121L - EACH |
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INC2022 | Scientific Laboratory Supplies | EACH | EUR 2288.25 |
Benchtop Incubator CLAD - 150L - EACH |
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INC2024 | Scientific Laboratory Supplies | EACH | EUR 2312.55 |
Benchtop Incubator CLAD - 200L - EACH |
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INC2026 | Scientific Laboratory Supplies | EACH | EUR 2949.75 |
BD720 Incubator Gravity 720L - EACH |
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INC3035 | Scientific Laboratory Supplies | EACH | EUR 8871.55 |
LMS Cooled Incubator 227L Series 3 - EACH |
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INC5010 | Scientific Laboratory Supplies | EACH | EUR 6862.23 |
LMS Cooled Incubator 290L Series 3 - EACH |
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INC5012 | Scientific Laboratory Supplies | EACH | EUR 7436.53 |
LMS Cooled Incubator 450L Series 3 - EACH |
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INC5014 | Scientific Laboratory Supplies | EACH | EUR 7427.7 |
LMS Cooled Incubator 600L Series 4 - EACH |
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INC5020 | Scientific Laboratory Supplies | EACH | EUR 8805.46 |
LMS Cooled Incubator 135L Series 2 - EACH |
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INC5324 | Scientific Laboratory Supplies | EACH | EUR 5269.05 |
LMS Cooled Incubator 200L Series 2 - EACH |
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INC5326 | Scientific Laboratory Supplies | EACH | EUR 4863.2 |
LMS Cooled Incubator 290L Series 2 - EACH |
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INC5328 | Scientific Laboratory Supplies | EACH | EUR 5618.7 |
LMS Cooled Incubator 420L Series 2 - EACH |
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INC5330 | Scientific Laboratory Supplies | EACH | EUR 6940.35 |
Series 3 CO2 incubator TC - EACH |
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INC0611 | Scientific Laboratory Supplies | EACH | EUR 17055.9 |
Series 3 CO2 incubator IR - EACH |
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INC0612 | Scientific Laboratory Supplies | EACH | EUR 21405.6 |
115L Incubator Shaker Cooled - EACH |
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SHA3008 | Scientific Laboratory Supplies | EACH | EUR 11207.7 |
MyTemp™ Mini CO2 Digital Incubator, 115V |
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H2300-HC2 | Benchmark Scientific | 1 each | EUR 4518.82 |
MyTemp™ Mini CO2 Digital Incubator, 230V |
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H2300-HC2-E | Benchmark Scientific | 1 PC | EUR 4518.82 |
Forced air cooled incubator, 150 Lt volume, |
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IC-150-R | Phoenix instrument | each | EUR 4285 |
LMS Cooled Incubator 410L XAL series - EACH |
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INC5016 | Scientific Laboratory Supplies | EACH | EUR 13109.85 |
LMS Cooled Incubator 600L XAL series - EACH |
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INC5018 | Scientific Laboratory Supplies | EACH | EUR 15819.3 |
Genorise® INC-C-1 Incubator |
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GR146002 | Genorise Scientific | - | Ask for price |
Prime Incubator Sts 100C - 30L - EACH |
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INC2048 | Scientific Laboratory Supplies | EACH | EUR 2019.6 |
Prime Incubator Sts 100C - 50L - EACH |
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INC2058 | Scientific Laboratory Supplies | EACH | EUR 2280.15 |
Prime Incubator Sts 100C - 75L - EACH |
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INC2224 | Scientific Laboratory Supplies | EACH | EUR 2304.45 |
Prime Incubator Sts 100C - 100L - EACH |
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INC2226 | Scientific Laboratory Supplies | EACH | EUR 2439.45 |
Prime Incubator Sts 100C - 125L - EACH |
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INC2228 | Scientific Laboratory Supplies | EACH | EUR 2650.05 |
Prime Incubator Sts 100C - 150L - EACH |
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INC2230 | Scientific Laboratory Supplies | EACH | EUR 2867.4 |
Prime Incubator Sts 100C - 200L - EACH |
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INC2232 | Scientific Laboratory Supplies | EACH | EUR 3414.15 |
LMS Incubator 76L Series 1A - EACH |
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INC5300 | Scientific Laboratory Supplies | EACH | EUR 4162.05 |
IKA KS4000i Shaking Incubator - EACH |
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SHA2600 | Scientific Laboratory Supplies | EACH | EUR 9782.1 |
C170i Incubator left side O2 - EACH |
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E6731001022 | Scientific Laboratory Supplies | EACH | EUR 14777.1 |
Incubator/Oven Base Mount - 6L - EACH |
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INC2242 | Scientific Laboratory Supplies | EACH | EUR 1491.75 |
Galaxy 48R CO2 Incubator 48L - EACH |
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INC6440 | Scientific Laboratory Supplies | EACH | EUR 7483.05 |
MyTemp Mini CO2 Digital Incubator 230V - EACH |
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INC2308 | Scientific Laboratory Supplies | EACH | EUR 6473.25 |
NEDD8 ultimate buster 1 |
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AP86284 | SAB | 1mg | EUR 2640 |
NEDD8 ultimate buster 1 |
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AP85555 | SAB | 1mg | EUR 2640 |
NEDD8 ultimate buster 1 |
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AP85935 | SAB | 1mg | EUR 2640 |
C170i CO2 Incubator Left Side - EACH |
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E6731000022 | Scientific Laboratory Supplies | EACH | EUR 12145.95 |
C170 CO2 Incubator Right Side - EACH |
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E6734000012 | Scientific Laboratory Supplies | EACH | EUR 10405.8 |
Extra Shelf for 40L Incubator - EACH |
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INC1503 | Scientific Laboratory Supplies | EACH | EUR 209.25 |
Extra Shelf for 70L Incubator - EACH |
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INC1504 | Scientific Laboratory Supplies | EACH | EUR 209.25 |
Extra Shelf for 130L Incubator - EACH |
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INC1505 | Scientific Laboratory Supplies | EACH | EUR 209.25 |
Extra Shelf for 45L Incubator - EACH |
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INC1510 | Scientific Laboratory Supplies | EACH | EUR 280.8 |
Extra Shelf for 180L Incubator - EACH |
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INC1511 | Scientific Laboratory Supplies | EACH | EUR 558.9 |
Incubator/Oven Base Mount - 18L - EACH |
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INC2244 | Scientific Laboratory Supplies | EACH | EUR 1640.25 |
Incubator/Oven Base Mount - 30L - EACH |
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INC2246 | Scientific Laboratory Supplies | EACH | EUR 1756.35 |
Incubator/Oven Base Mount - 40L - EACH |
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INC2248 | Scientific Laboratory Supplies | EACH | EUR 1935.9 |
Incubator/Oven Base Mount - 53L - EACH |
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INC2250 | Scientific Laboratory Supplies | EACH | EUR 2049.3 |
Incubator/Oven Base Mount - 73L - EACH |
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INC2252 | Scientific Laboratory Supplies | EACH | EUR 2370.6 |
Incubator/Oven Base Mount - 100L - EACH |
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INC2254 | Scientific Laboratory Supplies | EACH | EUR 2494.8 |
Incubator/Oven Base Mount - 121L - EACH |
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INC2256 | Scientific Laboratory Supplies | EACH | EUR 2488.05 |